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Catalog Number

LDG021PHM

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Specifications
- Species of Origin
  
  Human
  
  Expression system
  
  HEK293
- Affinity Tag
  
  His Tag (C-term)
  
  Buffer
  
  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
- Purity
  
  >90% as determined by SDS-PAGE analysis.
  
  Molecular weight
  
  The protein has a calculated MW of 80.48 kDa.
  The protein migrates as 75-100 kDa under reducing condition (SDS-PAGE analysis).
- Activity
  
  Measured by its ability to bind mouse c-Met in functional ELISA. Immobilized Recombinant mouse c-Met Protein at 2 μg/mL (100 μL/well) can bind Recombinant Human HGF Protein.
  
  Endotoxin level
  
  <1 EU per 1 μg of the protein by the LAL method.
- Form
  
  Lyophilized
Background
- Background
  
  Hepatocyte Growth Factor (HGF) activates c-Met receptor's tyrosine kinase cascade, influencing cell growth and motility. Originating from mesenchymal cells, HGF is a multifunctional cytokine that plays a central role in angiogenesis, tumorigenesis, and tissue regeneration. Initially secreted as an inactive polypeptide, HGF undergoes cleavage to become an active heterodimeric molecule. The gene exhibits alternative splicing, resulting in diverse isoforms.
  
  Synonyms
  
  Hepatocyte growth factor, HGF, Scatter factor, SF, HGF/SF, Hepatopoietin-A, HPTA, HGFB, Fibroblast-derived tumor cytotoxic factor, F-TCF, Lung fibroblast-derived mitogen, MET ligand, c-Met ligand, Prepro-hepatocyte growth factor
- Uniprot ID
  
  P14210
  
  Sequence Note
  
  Gln32-Ser728
Instruction
- Reconstitution
  
  It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
  
  Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
- Stability & Storage
  
  This product is stable after storage at:
  - -20°C for 12 months in lyophilized state from date of receipt.
  - -20°C or -80°C for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
Image

Activity analysis of recombinant Human HGF, His Tag, HEK293.
Measured by its ability to bind mouse c-Met in functional ELISA. Immobilized Recombinant mouse c-Met Protein at 2 μg/mL (100 μL/well) can bind Recombinant Human HGF Protein.

HPLC analysis of recombinant Human HGF, His Tag, HEK293.
The purity of Human HGF Protein is greater than 95% as determined by RP-HPLC

SDS-PAGE analysis of recombinant Human HGF.

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Datasheet & Documents

Datasheet

MSDS

Manual

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