Human HGF, His Tag, CHO

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Catalog Number

LDG187PHM

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• Specifications

  • Species of Origin

    Human

    Expression system

    CHO

  • Affinity Tag

    His Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 80.48 kDa.
    The protein migrates as 30-93 kDa under reducing condition due to glycosylation (SDS-PAGE analysis).

  • Activity

    Measured by its ability to bind mouse c-Met in functional ELISA. Immobilized Recombinant mouse c-Met Protein at 2 μg/mL (100 μL/well) can bind Recombinant Human HGF Protein.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    HGF is a multifunctional growth factor that binds to the c-Met receptor, regulating cell proliferation, motility, survival, and morphogenesis. It plays essential roles in liver regeneration, angiogenesis, tissue repair, and tumor invasion/metastasis.

    Synonyms

    Hepatocyte growth factor, SF (Scatter Factor), HPTA, HGFB, Hepatopoietin A, DF (Dispersion Factor), c-Met ligand

  • Uniprot ID

    P14210

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Activity analysis of recombinant Human HGF, His Tag, CHO.
  Measured by its ability to bind mouse c-Met in functional ELISA. Immobilized Recombinant mouse c-Met Protein at 2 μg/mL (100 μL/well) can bind Recombinant Human HGF Protein.

  SDS-PAGE analysis of recombinant Human HGF.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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