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Human FGF-8b, His Tag, E. coli
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  • Specifications
    • Species of Origin


      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)


      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 22.14 kDa.
      The protein migrates as 23 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is 1.4-3.8 ng/mL. The specific activity of recombinant human FGF-8b is > 2 x 10⁵ IU/mg.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form


  • Background
    • Background

      Fibroblast Growth Factors-8b (FGF-8b) is a 21.3 kDa member of the fibroblast Growth Factors with 183 amino acid residues. FGF-8 is an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. In embryogenesis function, FGF8 is necessary in limb, brain, eye and ear formation. FGF-8b having a more potent inductive activity due to higher affinity for Fgf receptors.


      Androgen-induced Growth Factors, AIGF, Heparin-binding Growth Factors 8, HBGF-8, Fibroblast Growth Factors 8

    • Uniprot ID


      Sequence Note


  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.


      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Human FGF-8b, His Tag, E. coli (LDG074PHE) induced 3T3 cell proliferation, with the ED50 at 3.51 ng/mL.

    Human FGF-8b, His Tag, E. coli (LDG074PHE) induced 3T3 cell proliferation, with the ED50 at 3.51 ng/mL.

    SDS-PAGE analysis of recombinant human FGF-8b.

    SDS-PAGE analysis of recombinant human FGF-8b.

    Total   (2)
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  • Datasheet & Documents

Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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