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Species of Origin
Human
Expression system
Escherichia coli
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Affinity Tag
His Tag (C-term)
Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>95% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 24.57 kDa.
The protein migrates as 24 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <31 ng/mL. The specific activity of recombinant human FGF-16 is > 3 x 10⁴ IU/mg.
Endotoxin level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
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Specifications
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Background
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Background
Fibroblast Growth Factors-16 (FGF-16) is a 23.8 kDa member of the fibroblast Growth Factors with 207 amino acid residues. FGF-16 is mainly expressed from melanocytes, fibroblasts. FGF-16 involved embryonic development, cell proliferation and cell differentiation, and is required for normal cardiomyocyte proliferation and heart development.
Synonyms
Fibroblast Growth Factors 16
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Uniprot ID
#O43320
Sequence Note
Met1-Arg207
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Publication
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Datasheet & Documents
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Disclaimer:For Research Use or Further Manufacturing Only.