Leadgene Leadgene
Human ENO1, His Tag, E. coli
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  • Specifications
    • Species of Origin


      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (N-term)


      Lyophilized from a 0.2 µm filtered solution of PBS with 7 mM MgSO₄, pH 7.2

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 47.85 kDa.
      The protein migrates as 55 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.



  • Background
    • Background

      Enolase 1 (ENO1) also named alpha-enolase which is a glycolytic enzyme. It could convert the NAD+ to NADH by producing two ATP. ENO1 is a homodimer which is composed by two isozymes of enolase (2 α, 2 ν, or 2 β). It also plays an important role in cancer model which can promote tumor cell proliferation and migration by the PI3K signaling pathway. ENO1 is a biomarker of prognostic and diagnostic cancer.


      Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Phosphopyruvate hydratase, Plasminogen-binding protein

    • Uniprot ID

      # P06733 2

      Sequence Note


  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.


      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human ENO1.

    SDS-PAGE analysis of recombinant human ENO1.

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  • Publication
  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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