Leadgene Leadgene
Human Complement C3b, His-Avi Tag, CHO
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Catalog Number

LDG166PHM

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  • Specifications
    • Species of Origin

      Human

      Expression system

      CHO

    • Affinity Tag

      His-Avi (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >90% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 65.5kDa.
      The protein migrates as 40-60 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      Complement C3b is a central protein fragment generated from the cleavage of complement component C3, playing a crucial role in the activation of the complement system. C3b functions as an opsonin, tagging pathogens for phagocytosis, and participates in the formation of C5 convertase. It is essential in innate immunity, pathogen clearance, and modulation of adaptive immune responses, making it a valuable tool in immunology and infectious disease research.

      Synonyms

      C3b fragment, Complement component 3b, Opsonic fragment, C3 beta chain, Beta chain of complement C3

    • Uniprot ID

      P05156

      Sequence Note

      Lys19-Val583

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    HPLC analysis of recombinant Human Complement C3b, His-Avi Tag, CHO. The purity of Human Complement C3b Protein is greater than 95% as determined by RP-HPLC.

    HPLC analysis of recombinant Human Complement C3b, His-Avi Tag, CHO.
    The purity of Human Complement C3b Protein is greater than 95% as determined by RP-HPLC.

    SDS-PAGE analysis of recombinant Human Complement C3b.

    SDS-PAGE analysis of recombinant Human Complement C3b.

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  • Datasheet & Documents
    MSDS
    Manual
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