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Human CD33, His Tag, HEK293
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Catalog Number

LDG065PHM

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  • Specifications
    • Species of Origin

      Human

      Expression system

      HEK293

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, 0.1 M Arginine, pH 7.4.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 27.67 kDa.
      The protein migrates as 38-50 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measured by its ability to support the adhesion of human red blood cells, with the ED50 being < 4 µg/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      CD33 is a myeloid cell surface antigen that regulates immune cell activity through inhibitory signaling. It is implicated in acute myeloid leukemia and serves as a therapeutic target.

      Synonyms

      Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67, CD33

    • Uniprot ID

      P20138

      Sequence Note

      Asp18-His259

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    HPLC analysis of recombinant Human CD33, His Tag, HEK293. The purity of Human CD33 Protein is greater than 95% as determined by RP-HPLC.

    HPLC analysis of recombinant Human CD33, His Tag, HEK293.
    The purity of Human CD33 Protein is greater than 95% as determined by RP-HPLC.

    Human CD33 Protein, His Tag, HEK293 (LDG065PHM) supports the adhesion of human red blood cells, with the ED50 being &lt; 4 µg/mL.

    Human CD33 Protein, His Tag, HEK293 (LDG065PHM) supports the adhesion of human red blood cells, with the ED50 being < 4 µg/mL.

    SDS-PAGE analysis of recombinant Human CD33.

    SDS-PAGE analysis of recombinant Human CD33.

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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