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Leadgene Leadgene
Human CD28 ECD, His-SUMO Tag, HEK293
Catalog Number

LDG014PHM

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  • Specifications
    • Species of Origin

      Human

      Expression system

      HEK293

    • Affinity Tag

      His-SUMO Tag (N-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 28.61 kDa.
      The protein migrates as 48-62 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin level

      <1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      CD28, a well-known costimulatory receptor expressed on T cells, is a type I transmembrane glycoprotein of the Ig superfamily. When the T cells receive the first signal delivered by APCs (antigen-presenting cells), it initiates signaling cascades, which favor T cell survival and proliferation. It has been demonstrated that patients with lung cancer who responded to PD-1 therapy had more CD28+ T cells, suggesting that CD28 may predict treatment response.

      Synonyms

      TP44

    • Uniprot ID

      # P10747

      Sequence Note

      Asn19-Pro152

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of Human CD28 ECD.

    SDS-PAGE analysis of Human CD28 ECD.

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  • Datasheet & Documents
    MSDS
    Manual
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