Human CD276, His Tag, CHO

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Catalog Number

LDG052PHM

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    CHO

  • Affinity Tag

    His Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 24.12 kDa.
    The protein migrates as 26-45 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measured by its ability to bind Human CD276 Antibody in functional ELISA. Immobilized Recombinant human CD276 Protein at 2 μg/mL (50 μL/well) can bind Human CD276 Antibody.

    Form

    Lyophilized

• Background

  • Background

    Human CD276, also known as B7-H3, is a member of the B7 family of immune checkpoint proteins and plays a role in the regulation of T cell-mediated immune responses. CD276 is highly expressed in various tumor types and is associated with tumor immune evasion, making it a target of interest in cancer immunotherapy. The Human CD276 protein, when expressed in CHO (Chinese Hamster Ovary) cells, is often tagged with a His (histidine) tag to facilitate its purification and detection. This recombinant CD276-His fusion protein is used in research focused on understanding its role in cancer and developing therapeutic strategies.

    Synonyms

    CD276 antigen, 4Ig-B7-H3, B7 homolog 3, B7-H3, Costimulatory molecule, CD276

  • Uniprot ID

    NCBI: NP_001316557.1

    Sequence Note

    Leu29-Pro245

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  HPLC analysis of recombinant Human CD276, His Tag, CHO.
  The purity of Human CD276 Protein is greater than 90% as determined by RP-HPLC.

  SDS-PAGE analysis of recombinant Human CD276.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.