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Leadgene Leadgene
Human CCL2, His Tag, E. coli
Catalog Number

LDG164PHE

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  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (N-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 9.49 kDa.
      The protein migrates as 11 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to chemoattract BaF3 cells transfected with human CCR2A. The ED₅₀ for this effect is <20 ng/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      C-C Motif Chemokine Ligand 2 (CCL2) is an 8.44 kDa cytokine with 76 amino acid residues and is also known as monocyte chemoattractant protein 1 (MCP1). The CCL2 secreted from monocytes, macrophages, and dendritic cells. Upon binding with CCR4, it regulates many biological functions, such as recruitments of monocytes, memory T cells, and dendritic cells, response to TNF-α and Interferon-γ, angiogenesis, cellular homeostasis, and cytoskeleton organization.

      Synonyms

      Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, MCAF, Monocyte chemotactic protein 1, MCP-1, Monocyte secretory protein JE, Small-inducible cytokine A2, C-C motif chemokine 2

    • Uniprot ID

      # P13500 1

      Sequence Note

      Gln24-Thr99

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human CCL2.

    SDS-PAGE analysis of recombinant human CCL2.

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  • Datasheet & Documents
    MSDS
    Manual
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