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Catalog Number

LDG094PHM

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Specifications
- Species of Origin
  
  Human
  
  Expression system
  
  CHO
- Affinity Tag
  
  His Tag (C-term)
  
  Buffer
  
  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
- Purity
  
  >95% as determined by SDS-PAGE analysis.
  
  Molecular weight
  
  The protein has a calculated MW of 14.56 kDa.
  The protein migrates as 20-55 kDa under reducing condition (SDS-PAGE analysis).
- Activity
  
  Measured by its ability to bind mouse HVEM in functional ELISA. Immobilized Recombinant mouse HVEM Protein at 0.5 μg/mL (50 μL/well) can bind Recombinant Human BTLA Protein.
  
  Endotoxin level
  
  <0.1 EU per 1 μg of the protein by the LAL method.
- Form
  
  Lyophilized
Background
- Background
  
  BTLA (B- and T-lymphocyte attenuator) is an inhibitory receptor expressed on lymphocytes, playing a role in regulating immune responses.
  
  Synonyms
  
  B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein, CD272
- Uniprot ID
  
  Q7Z6A9
  
  Sequence Note
  
  Lys 31 - Ser 150
Instruction
- Reconstitution
  
  It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
  
  Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
- Stability & Storage
  
  This product is stable after storage at:
  - -20°C for 12 months in lyophilized state from date of receipt.
  - -20°C or -80°C for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
Image

Activity analysis of recombinant Human BTLA, His Tag, CHO.
Measured by its ability to bind mouse HVEM in functional ELISA. Immobilized Recombinant mouse HVEM Protein at 0.5 μg/mL (50 μL/well) can bind Recombinant Human BTLA Protein.

HPLC analysis of recombinant Human BTLA, His Tag, CHO.
The purity of Human BTLA Protein is greater than 95% as determined by RP-HPLC.

SDS-PAGE analysis of recombinant Human BTLA Protein.

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Datasheet & Documents

Datasheet

MSDS

Manual

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