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Leadgene Leadgene
Human BMP-12, His Tag, E. coli
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Catalog Number

LDG110PHE

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  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 4.5.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 14.95 kDa.
      The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is < 2 μg/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Bone Morphogenetic Protein-12 (BMP-12), known as Growth differentiation factor 7 (GDF7), is an extracellular multifunctional cytokine that is also a member of the TGFβ family. BMP-12 can bind with the TGFβ receptor and trigger SMAD protein signal transduction. BMP-12 is in a signaling center called the roof plate in the embryo's developing nervous system, where it sends signals to induce the generation of sensory neurons.

      Synonyms

      Growth/differentiation factor 7

    • Uniprot ID

      #Q7Z4P5

      Sequence Note

      Lys352-Arg450

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Human BMP-12, His Tag, E. coli (LDG110PHE) induced alkaline phosphatase production by ATDC5 cells, with the ED50 at 111.8 ng/mL.

    Human BMP-12, His Tag, E. coli (LDG110PHE) induced alkaline phosphatase production by ATDC5 cells, with the ED50 at 111.8 ng/mL.

    SDS-PAGE analysis of recombinant human BMP-12.

    SDS-PAGE analysis of recombinant human BMP-12.

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  • Datasheet & Documents
    MSDS
    Manual
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