Leadgene Leadgene
Human BAFF, His Tag, E. coli
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  • Specifications
    • Species of Origin


      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)


      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 17.98 kDa.
      The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce IL-8 secretion in human PBMCs. The ED₅₀ for this effect is <0.5 ng/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form


  • Background
    • Background

      BAFF also known as BLYS, TALL-1 and TNFSF13B, which belongs to tumor necrosis factor family. BAFF is a 31.2 kDa type II transmembrane protein containing 285 residues that predominantly produced by myeloid cells. BAFF has been demonstrated to activate the survival of B-cells and the B-cell response by binding to BAFFR/BR3. Additionally, BAFF also takes part in regulating B and T cell function via forming two ligands-two receptors pathway through sharing TNFRSF13B/TACI and TNFRSF17/BCMA receptors with APRIL.


      B-cell-activating factor, BAFF, B lymphocyte stimulator, BLyS, Dendritic cell-derived TNF-like molecule, TNF- and APOL-related leukocyte expressed ligand 1, TALL-1, CD257, Tumor necrosis factor ligand superfamily member 13B

    • Uniprot ID


      Sequence Note


  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.


      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Human BAFF, His Tag, E. coli (LDG128PHE) induced IL-8 secretion in human PBMCs, with the ED50 at 0.4031 ng/mL.

    Human BAFF, His Tag, E. coli (LDG128PHE) induced IL-8 secretion in human PBMCs, with the ED50 at 0.4031 ng/mL.

    SDS-PAGE analysis of recombinant human BAFF.

    SDS-PAGE analysis of recombinant human BAFF.

    Total   (2)
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  • Datasheet & Documents

Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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