Language
EN
Leadgene Leadgene
Human BAFF, His Tag, E. coli
More Images
Catalog Number

LDG128PHE

More Documents
Loading package & price...
  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 17.98 kDa.
      The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce IL-8 secretion in human PBMCs. The ED₅₀ for this effect is <0.5 ng/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      BAFF also known as BLYS, TALL-1 and TNFSF13B, which belongs to tumor necrosis factor family. BAFF is a 31.2 kDa type II transmembrane protein containing 285 residues that predominantly produced by myeloid cells. BAFF has been demonstrated to activate the survival of B-cells and the B-cell response by binding to BAFFR/BR3. Additionally, BAFF also takes part in regulating B and T cell function via forming two ligands-two receptors pathway through sharing TNFRSF13B/TACI and TNFRSF17/BCMA receptors with APRIL.

      Synonyms

      B-cell-activating factor, BAFF, B lymphocyte stimulator, BLyS, Dendritic cell-derived TNF-like molecule, TNF- and APOL-related leukocyte expressed ligand 1, TALL-1, CD257, Tumor necrosis factor ligand superfamily member 13B

    • Uniprot ID

      #Q9Y275

      Sequence Note

      Ala134-Leu285

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Human BAFF, His Tag, E. coli (LDG128PHE) induced IL-8 secretion in human PBMCs, with the ED50 at 0.4031 ng/mL.

    Human BAFF, His Tag, E. coli (LDG128PHE) induced IL-8 secretion in human PBMCs, with the ED50 at 0.4031 ng/mL.

    SDS-PAGE analysis of recombinant human BAFF.

    SDS-PAGE analysis of recombinant human BAFF.

    1
    /
    2
    Total   (2)
  • Review

    Help others learn more about this product. Use the link below to share your experience.

  • Publication

    There are currently no publications. Use the link below to let us know.

  • Datasheet & Documents
    MSDS
    Manual
    1
    /
    1

Disclaimer:For Research Use or Further Manufacturing Only.

This site uses cookies as described in our Privacy Policy in order to improve your user experience and deliver customized advertising to you. Click “I Agree” to consent and continue.