Leadgene Leadgene
Human BACE1, His Tag, CHO
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Catalog Number

LDG130PHM

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  • Specifications
    • Species of Origin

      Human

      Expression system

      CHO

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 49.25 kDa.
      The protein migrates as 60-75 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      BACE1 cleaves amyloid precursor protein, initiating amyloid-beta production, and is a therapeutic target for Alzheimer’s disease.

      Synonyms

      Beta-secretase 1, EC:3.4.23.46, Aspartyl protease 2, ASP2, Asp 2, Beta-site amyloid precursor protein cleaving enzyme 1, Beta-site APP cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2

    • Uniprot ID

      P56817

      Sequence Note

      Thr 22-Thr 457

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    HPLC analysis of recombinant Human BACE1, His Tag, CHO. The purity of Human BACE1 Protein is greater than 95% as determined by RP-HPLC.

    HPLC analysis of recombinant Human BACE1, His Tag, CHO.
    The purity of Human BACE1 Protein is greater than 95% as determined by RP-HPLC.

    SDS-PAGE analysis of recombinant Human BACE1.

    SDS-PAGE analysis of recombinant Human BACE1.

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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