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Leadgene Leadgene
Human APRIL, His Tag, E. coli
Catalog Number

LDG124PHE

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  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 17.29 kDa.
      The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measured by its ability to induce cell death in Jurkat cells. The ED₅₀ for this effect is 2.6-4.0 μg/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      APRIL is a member of tumor necrosis factor ligand superfamily that expressed by stromal tissue, macrophages, and T cells. APRIL is a 27.4 kDa protein containing 250 residues, which plays a critical role in modulating tumor progression. Besides, APRIL has been demonstrated to involve in regulating B and T cell survival, proliferation and differentiation via binding with TNFRSF13B/TACI and TNFRSF17/BCMA.

      Synonyms

      A proliferation-inducing ligand, APRIL, TNF- and APOL-related leukocyte expressed ligand 2, TALL-2, TNF-related death ligand 1, TRDL-1, CD256, Tumor necrosis factor ligand superfamily member 13, TNFL13

    • Uniprot ID

      #AAQ91388

      Sequence Note

      Ala1-Leu146

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human APRIL.

    SDS-PAGE analysis of recombinant human APRIL.

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  • Datasheet & Documents
    MSDS
    Manual
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