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Leadgene Leadgene
Human Annexin V, His Tag, E. coli
Catalog Number

LDG058PHE

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  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 36.75 kDa.
      The protein migrates as 37 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      Annexin V predicts a molecular mass of 36.8 kDa. is commonly used to detect apoptotic cells by its ability to bind to phosphatidylserine, a marker of apoptosis when it is on the outer leaflet of the plasma membrane. Annexin V forms a shield around negatively charged phospholipid molecules. The formation of an annexin V shield blocks the entry of phospholipids into coagulation reactions.

      Synonyms

      Anchorin CII, Annexin V, Calphobindin I , CPB-I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4 , PP4, Placental anticoagulant protein I , PAP-I, Vascular anticoagulant-alpha , VAC-alpha

    • Uniprot ID

      #P08758

      Sequence Note

      Met1-Asp320

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human Annexin V.

    SDS-PAGE analysis of recombinant human Annexin V.

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  • Datasheet & Documents
    MSDS
    Manual
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