Leadgene Leadgene
HCoV-229E Nucleocapsid Protein, His Tag, E. coli
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  • Specifications
    • Species of Origin

      Human coronavirus 229E

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)


      Lyophilized from a 0.2 µm filtered solution of PBS containing 500 mM NaCl, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 44.28 kDa.
      The protein migrates as 45 kDa under reducing condition (SDS-PAGE analysis).

    • Form


  • Background
    • Background

      There are seven human coronaviruses have been identified. The common human coronaviruses are four groups, known as 229E (α coronavirus), NL63 (α coronavirus), OC43 (β coronavirus) and HKU1 (β coronavirus). Because the crown-like spikes on the surface of virus, they are named for coronaviruses. HCoVs cause the respiratory tract diseases, especially severe in infants and the elderly. The spike protein controled the infection of target cells and it facilitated entry into cells by binding cellular receptors.


      Nucleoprotein, Nucleocapsid protein, NC Protein N

    • Uniprot ID


      Sequence Note


  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.


      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant Human Coronavirus (229E) nucleocapsid protein.

    SDS-PAGE analysis of recombinant Human Coronavirus (229E) nucleocapsid protein.

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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