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Leadgene Leadgene
Enterovirus A71 (EV-A71) VP1 Protein, His Tag, E. coli
Catalog Number

LDG029PVE

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  • Specifications
    • Species of Origin

      Enterovirus A71

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 33.76 kDa.
      The protein migrates as 35 kDa under reducing condition (SDS-PAGE analysis).

    • Form

      Lyophilized

  • Background
    • Background

      Enterovirus A71 (EV-A71), a positive-strand RNA virus of the Picornaviridae family, is a persisting global concern. EV-A71 is primarily transmitted through the fecal-oral route, causing hand, foot, and mouth disease (HFMD), severe central nervous system complications, neural damage, and even life-threatening. VP1 is a structural capsid protein consisting of 297 amino acids, promoting infection by binding to specific receptors on the surface of host cells, including P-selectin glycoprotein ligand 1 (PSGL-1), scavenger receptor B2 (SCARB2), annexin II protein, sialylated glycan, and heparan sulfate.

      Synonyms

      P1D, Virion protein 1

    • Uniprot ID

      #BDI13007.1 (GeneBank ID)

      Sequence Note

      Gly1-Ser297

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of Enterovirus A71 (EV-A71) VP1 protein.

    SDS-PAGE analysis of Enterovirus A71 (EV-A71) VP1 protein.

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  • Datasheet & Documents
    MSDS
    Manual
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