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Leadgene Leadgene
Anti-TGF Beta 1 Antibody [Clone 51-3]
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Catalog Number

LDG0047YA

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  • Overview

    Description

    Transforming growth factor beta (TGF-β), a polypeptide member of the transforming growth factor beta superfamily of cytokines that includes three isoforms, is involved in various biological processes. TGF-β1, the most abundant TGF-β isoform, is a secreted protein that plays important role in cell proliferation, differentiation and apoptosis and is involved in wound healing, inflammatory processes and prevention of tumor growth.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-10000
    IFA: 1:400-1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG1

      Clone Name

      clone 51-3

    • Immunogen

      TGF Beta 1

      Reactivity

      Human

    • Application

      ELISA, WB, IFA, FACS

      Conjugation

      Unconjugated

    • Concentration

      1 mg/mL

      Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Specificity

      TGF Beta 1

      Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    Immunofluorescence analysis of Anti-TGF Beta 1 Antibody [Clone 51-3] 293T cells were respectively transfected with TGF beta 1, beta 2 and beta 3 plasmid and fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with mouse anti-TGF beta 1 monoclonal antibody (1:400) followed by secondary antibodies (goat anti-Mouse IgG- iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    Immunofluorescence analysis of Anti-TGF Beta 1 Antibody [Clone 51-3]
    293T cells were respectively transfected with TGF beta 1, beta 2 and beta 3
    plasmid and fixed in 4% PFA, permeabilized with PBS containing 0.1%
    Triton X-100. Cells were stained with mouse anti-TGF beta 1 monoclonal
    antibody (1:400) followed by secondary antibodies (goat anti-Mouse IgG-
    iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342
    (Blue).

    FACS analysis of Anti-TGF Beta 1 Antibody [Clone 51-3] 293T cells (black) were respectively transfected with TGF beta 1 (red), beta 2 (blue) and beta 3 (green) plasmid and stained with mouse anti-TGF beta 1 monoclonal antibody at 1 μg/ml.

    FACS analysis of Anti-TGF Beta 1 Antibody [Clone 51-3]
    293T cells (black) were respectively transfected with TGF beta 1 (red), beta
    2 (blue) and beta 3 (green) plasmid and stained with mouse anti-TGF beta 1
    monoclonal antibody at 1 μg/ml.

    ELISA titration of Anti-TGF Beta 1 Antibody [Clone 51-3] Titration curve of anti-TGF beta 1 antibody in ELISA. Red: TGF beta 1; Black: BSA (negative control).

    ELISA titration of Anti-TGF Beta 1 Antibody [Clone 51-3]
    Titration curve of anti-TGF beta 1 antibody in ELISA. Red: TGF beta 1;
    Black: BSA (negative control).

    Western blotting analysis of Anti-TGF Beta 1 Antibody [Clone 51-3] Recombinant proteins and TGF beta 1, 2 or 3 transfected 293T cell lysates were stained with mouse anti-TGF beta 1 monoclonal antibody at 1:2000 dilution. Lane 1: recombinant TGF beta 1 protein (25 ng). Lane 2: recombinant TGF beta 2 protein (25 ng). Lane 3: recombinant TGF beta 3 protein (25 ng). Lane 4: TGF beta 1 transfected 293T cell lysates (30 μg). Lane 5: TGF beta 2 transfected 293T cell lysates (30 μg). Lane 6: TGF beta 3 transfected 293T cell lysates (30 μg).

    Western blotting analysis of Anti-TGF Beta 1 Antibody [Clone 51-3]
    Recombinant proteins and TGF beta 1, 2 or 3 transfected 293T cell lysates
    were stained with mouse anti-TGF beta 1 monoclonal antibody at 1:2000
    dilution. Lane 1: recombinant TGF beta 1 protein (25 ng). Lane 2:
    recombinant TGF beta 2 protein (25 ng). Lane 3: recombinant TGF beta 3
    protein (25 ng). Lane 4: TGF beta 1 transfected 293T cell lysates (30 μg).
    Lane 5: TGF beta 2 transfected 293T cell lysates (30 μg). Lane 6: TGF beta
    3 transfected 293T cell lysates (30 μg).

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  • Datasheet & Documents
    MSDS
    Manual
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