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Leadgene Leadgene
Anti-SARS-CoV & CoV-2 NP IgG Antibody
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Catalog Number

LDG0076YA

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  • Overview

    Description

    Human anti-SARS-CoV & CoV-2 NP antibody recognize human SARS Coronavirus Nucleocapsid protein (NP). Coronavirus NP localize to the cytoplasm and the nucleolus in both Virus-like particle (VLP) infected primary cells and in cells transfected with NP plasmid. NP has abundant expression in coronavirus and is a highly immunogenic phosphoprotein. NP is also conserved in sequence. Due to these characteristic above, the NP is an ideal marker for diagnosis.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-5000
    IFA: 1:500-1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Clonality

      Recombinant Human IgG

      Isotype

      IgG1

    • Reactivity

      SARS-CoV & CoV-2

      Application

      ELISA, WB, IFA

    • Conjugation

      Unconjugated

      Concentration

      1 mg/mL

    • Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

      Specificity

      Nucleocapsid protein

    • Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    Immunofluorescence analysis of Human anti-SARS-CoV & CoV-2 NP Antibody (IgG) (1:500)

    Immunofluorescence analysis of Human anti-SARS-CoV & CoV-2 NP Antibody (IgG) (1:500)

    Multi-cycle kinetic analysis of Human anti-SARS-CoV & CoV-2 NP Antibody (IgG) to Human SARS-CoV-2 Nucleocapsid protein by using Biacore T200.

    Multi-cycle kinetic analysis of Human anti-SARS-CoV & CoV-2 NP Antibody (IgG) to Human
    SARS-CoV-2 Nucleocapsid protein by using Biacore T200.

    ELISA titration of Human anti-SARS-CoV & CoV-2 NP Antibody (IgG)

    ELISA titration of Human anti-SARS-CoV & CoV-2 NP Antibody (IgG)

    Western blotting analysis of Human anti-SARS-CoV & CoV-2 NP antibody (IgG) (1:1000) Lane 1 : SARS-CoV-2 NP Lane 2 : SARS-CoV NP Lane 3 : MERS-CoV NP Lane 4 : HCoV-NL63 NP

    Western blotting analysis of Human anti-SARS-CoV & CoV-2 NP antibody (IgG) (1:1000)
    Lane 1 : SARS-CoV-2 NP
    Lane 2 : SARS-CoV NP
    Lane 3 : MERS-CoV NP
    Lane 4 : HCoV-NL63 NP

    Western blotting analysis of Human anti-SARS-CoV & CoV-2 NP antibody (IgG) (1:1000) Lane 1: 293T control lysate Lane 2: SARS-CoV-2 VLP

    Western blotting analysis of Human anti-SARS-CoV & CoV-2 NP antibody (IgG) (1:1000)
    Lane 1: 293T control lysate
    Lane 2: SARS-CoV-2 VLP

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  • Datasheet & Documents
    MSDS
    Manual
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