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Leadgene Leadgene
Anti-SARS-CoV-2 Spike Antibody [Clone LGSV201]
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Catalog Number

LDG0086YA

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  • Overview

    Description

    Human anti-SARS-CoV-2 Spike Antibody [CR3022] recognizes human SARS-CoV and CoV-2 Spike protein with high affinity. The binding site is amino acids 318-510 (RBD, Receptor Binding Domain) in the S1 subunit of the spike protein. Coronavirus spike protein conducts the process that interacts with cellular receptor and membrane fusion to allow virus entering into target cells. Spike protein also can be used to define the specificity of the virus and be used as a critical target for vaccine design. The glycosylated spike protein can be detected in the virus-infected cell and cell culture medium. The RBD is responsible for recognizing the cell surface receptor.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB:1: 1000-5000
    IFA:1: 500-1000
    FACS: Assay dependent


    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG1

      Clone Name

      clone LGSV201

    • Immunogen

      SARS-CoV-2 VLP

      Reactivity

      SARS-CoV & CoV-2

    • Application

      ELISA, WB, IFA

      Conjugation

      Unconjugated

    • Concentration

      1 mg/mL

      Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Specificity

      Spike protein

      Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    Western blotting analysis of Mouse anti-SARS-CoV-2 Spike mAb (1:5000) Lane: SARS-CoV-2 VLP

    Western blotting analysis of Mouse anti-SARS-CoV-2 Spike mAb (1:5000)
    Lane: SARS-CoV-2 VLP

    LDG0086YAWB01 Western blotting analysis of Mouse anti-SARS-CoV-2 Spike mAb (1:5000)Lane 1: SARS-CoV-2 S1-RBDLane 2: SARS-CoV-2 S1Lane 3: SARS-CoV S1-RBDLane 4: SARS-CoV S1Lane 5: MERS-CoV S1-RBD

    LDG0086YAWB01 Western blotting analysis of Mouse anti-SARS-CoV-2 Spike mAb (1:5000)Lane 1: SARS-CoV-2 S1-RBDLane 2: SARS-CoV-2 S1Lane 3: SARS-CoV S1-RBDLane 4: SARS-CoV S1Lane 5: MERS-CoV S1-RBD

    Immunofluorescence analysis of Mouse anti-SARS-CoV-2 Spike mAb (1:1000)

    Immunofluorescence analysis of Mouse anti-SARS-CoV-2 Spike mAb (1:1000)

    ELISA titration of Mouse anti-SARS-CoV-2 Spike mAb

    ELISA titration of Mouse anti-SARS-CoV-2 Spike mAb

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  • Datasheet & Documents
    MSDS
    Manual
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