Description
Interleukin-6 (IL-6) is a pleiotropic cytokine produced by activated immune cells and stromal cells, has proinflammatory and anti-inflammatory properties. This protein plays a crucial role in inflammation, immune response and hematopoiesis. It induces not only synthesis of acute phase proteins, but also promotes alternative activation of macrophages.
Product Note
Recognize IL-6 in ELISA, when clone 9D9 antibody was paired with Anti-IL-6 Antibody [clone 3-5] (cat. LDG0180YA).
Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-5000
IFA: 1:500-1000
FACS: Assay dependent
Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.