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Leadgene Leadgene
Anti-IL-33 Antibody [Clone 7E8]
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Catalog Number

LDG0033YA

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  • Overview

    Description

    Interleukin-33 (IL-33), a member of the IL-1 family, is a nuclear alarmin cytokine expressed by various cell types. Unlike most other cytokines, IL-33 lacks a secretory signal peptide, and thus it is passively released during cell damage or tissue injury. IL-33 plays a crucial role in type-2 innate immunity through interaction with the ST2 receptor.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-5000
    IFA: 1:400-1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG2a

      Clone Name

      clone 7E8

    • Immunogen

      Interleukin 33

      Reactivity

      Human

    • Application

      ELISA, WB, IFA, FACS

      Conjugation

      Unconjugated

    • Concentration

      1 mg/mL

      Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Specificity

      Interleukin 33

      Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    Immunofluorescence analysis of Anti-IL-33 Antibody [Clone 7E8] HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with mouse anti-IL-33 monoclonal antibody (1:400) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    Immunofluorescence analysis of Anti-IL-33 Antibody [Clone 7E8]
    HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
    Triton X-100. Cells were stained with mouse anti-IL-33 monoclonal antibody
    (1:400) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
    1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    FACS analysis of Anti-IL-33 Antibody [Clone 7E8] HeLa cells were stained with mouse anti-IL-33 monoclonal antibody at 1 μg/ml (red) and without antibody control (black).

    FACS analysis of Anti-IL-33 Antibody [Clone 7E8]
    HeLa cells were stained with mouse anti-IL-33 monoclonal antibody at 1
    μg/ml (red) and without antibody control (black).

    ELISA titration of Anti-IL-33 Antibody [Clone 7E8] Titration curve of anti-IL-33 antibody in ELISA. Red: IL-33; Black: BSA (negative control).

    ELISA titration of Anti-IL-33 Antibody [Clone 7E8]
    Titration curve of anti-IL-33 antibody in ELISA. Red: IL-33; Black: BSA
    (negative control).

    Western blotting analysis of Anti-IL-33 Antibody [Clone 7E8] Recombinant IL-33 protein (50 ng) was stained with mouse anti-IL-33 monoclonal antibody at 1:5000 dilution.

    Western blotting analysis of Anti-IL-33 Antibody [Clone 7E8]
    Recombinant IL-33 protein (50 ng) was stained with mouse anti-IL-33
    monoclonal antibody at 1:5000 dilution.

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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