Language
EN
Leadgene Leadgene
Anti-His Tag Antibody [Clone 14-4]
Catalog Number

LDG0001YB

More Documents
Loading package & price...
  • Overview

    Description

    Mouse anti-His tag mAb is specific to 6X His tag, which can be applied to several immunoassays, including ELISA, WB, IP, IFA, and FACS. This antibody has been identified in several sources (bacteria, yeast, and mammalian cells) of His tag fusion protein.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-5000
    IP: 1:500-2000
    IFA: 1:500-1000
    FACS: 1:500-1000

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG1

      Clone Name

      clone 14-4

    • Immunogen

      6X His tag fusion protein

      Application

      ELISA, WB, IP, IFA, FACS

    • Conjugation

      Unconjugated

      Concentration

      1 mg/mL

    • Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

      Specificity

      Recognizes 6X His tag on either the N- or C-terminus of recombinant fusion protein.

    • Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    Western blotting analysis of anti-His mAb (1:5000) Lane 1: 293T whole cell lysate-untransfected Lane 2: 293T whole cell lysate-transfected Lysate/proteins at 20 ug per lane

    Western blotting analysis of anti-His mAb (1:5000)
    Lane 1: 293T whole cell lysate-untransfected
    Lane 2: 293T whole cell lysate-transfected
    Lysate/proteins at 20 ug per lane

    1
    /
    1
    Total   (1)
  • Review

    Help others learn more about this product. Use the link below to share your experience.

  • Publication
  • Datasheet & Documents
    MSDS
    Manual
    1
    /
    1

Disclaimer:For Research Use or Further Manufacturing Only.

This site uses cookies as described in our Privacy Policy in order to improve your user experience and deliver customized advertising to you. Click “I Agree” to consent and continue.