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Leadgene Leadgene
Anti-HDGF Antibody [Clone 83-5]
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Catalog Number

LDG0040YA

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  • Overview

    Description

    Hepatoma-derived growth factor (HDGF;also known as high mobility group protein 1-like 2, HMG-1L2) is a heparin-binding protein that is ubiquitously expressed in normal tissues and abnormally expressed in various malignancies. HDGF has various intracellular functions and localizes to the nucleus, and thus it is characterized as a mitogen for many cell types. As its promoting growth, suppressing differentiation and exhibiting angiogenic properties, HDGF plays a critical role in tumor progression.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-10000
    IFA: 1:200-1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG1

      Clone Name

      clone 83-5

    • Immunogen

      HDGF

      Reactivity

      Human

    • Application

      ELISA, WB, IFA, FACS

      Conjugation

      Unconjugated

    • Concentration

      1 mg/mL

      Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Specificity

      HDGF

      Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    Immunofluorescence analysis of Anti-HDGF Antibody [Clone 83-5] Cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with anti-HDGF monoclonal antibody (1:200) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:200, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    Immunofluorescence analysis of Anti-HDGF Antibody [Clone 83-5]
    Cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton
    X-100. Cells were stained with anti-HDGF monoclonal antibody (1:200)
    followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:200,
    green) and cell nuclei were stained with Hoechst 33342 (Blue).

    Western blotting analysis of Anti-HDGF Antibody [Clone 83-5] Tissue lysate was stained with anti-HDGF monoclonal antibody at 1:5000 dilution. Lane 1: Mouse liver lysate (50 μg). Lane 2: Rat liver lysate (50 μg).

    Western blotting analysis of Anti-HDGF Antibody [Clone 83-5]
    Tissue lysate was stained with anti-HDGF monoclonal antibody at 1:5000
    dilution. Lane 1: Mouse liver lysate (50 μg). Lane 2: Rat liver lysate (50 μg).

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  • Datasheet & Documents
    MSDS
    Manual
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