Leadgene Leadgene
Anti-EGFP Antibody Sepharose Purification Kit
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  • Overview


    Anti-EGFP sepharose, is consist of an EGFP VHH coupled to NHS-sepharose. It is an efficient technique for isolating recombinant proteins or mammalian expression proteins. The EGFP epitope system relies on a VHH EGFP recombinant antibody, which is able to react with N- and C- terminal GFP tagged and EGFP tagged fusion proteins. This product can be used for the immuneprecipitation or immune affinity purification.
    The purified antibody is immobilized at 3-5 mg antibody per mL of 50% slurry and this kit allows a rapid and efficient affinity purification of active EGFP fusion proteins. The affinity resin allows an efficient binding of EGFP fusion proteins without the need for preliminary steps and calibrations. The affinity bound EGFP fusion proteins can be efficiently eluted from the resin by acid condition. The eluted proteins can be used for characteristic analysis.

    Product Note

    The optimal protein concentration of the cell lysate should be determined by the end user.

  • Specifications
    • Clonality



      Immunoprecipitation, Immunoaffinity Purification

    • Conjugation



      Recognizes GFP tag and EGFP tag on either the N- or C-terminus of the recombinant fusion protein.

    • Sample Type

      Cell lysate

      Binding capacity

      The binding and elution capacity of 1 mL settled Anti-EGFP sepharose may be vary, commonly more than 1 mg GFP or EGFP fusion proteins.

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product

      Please refer to product manual for storage constructions

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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