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Catalog Number

LDG0007YA

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Overview

Description

Dengue virus belongs to the family Flaviviridae, genus Flavivirus and consists of four distinct serotypes (DENV1 to 4). Dengue virus is transmitted by mosquitos majorly of species Aedes aegypti and Aedes Albopictus that widespread around tropics and subtropics. NS5 is the largest and the most conserved protein of Dengue virus, acting as both a RNA-dependent RNA polymerase and a RNA methyltransferase enzyme. The NS5 proteins are important for viral replication and can down-regulate the host immune interferon response.

Product Note

Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-5000
IFA: 1:500-1000
FACS: Assay dependent

Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Specifications
- Host
  
  Mouse
  
  Clonality
  
  Monoclonal
- Isotype
  
  IgG2b
  
  Clone Name
  
  clone 37-2
- Immunogen
  
  Dengue virus NS5
  
  Reactivity
  
  Dengue virus
- Application
  
  ELISA, WB, IFA, FACS
  
  Conjugation
  
  Unconjugated
- Concentration
  
  1 mg/mL
  
  Buffer
  
  Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.
- Specificity
  
  NS5
  
  Form
  
  Liquid
Instruction
- Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  Stability & Storage
  
  This product is stable after storage at:
  - 2-8°C for 2 weeks under sterile conditions from date of receipt.
  - -20°C or -80°C for 12 months under sterile conditions from date of receipt.
  Avoid repeated freeze/thaw cycles.
  Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.
Image

Immunofluorescence analysis of Anti-DENV NS5 Antibody [Clone 37-2]
Control Vero cells (uninfected) and Virus (DENV 1,2,4) infection Vero cells
were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-
100. Cells were stained with mouse anti-DENV NS5 monoclonal antibody
followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400,
green) and cell nuclei were stained with Hoechst 33342 (Blue).

ELISA titration of Anti-DENV NS5 Antibody [Clone 37-2]
Titration curve of anti-DENV NS5 antibody in ELISA. Red: NS5; Black: BSA
(negative control).

Western blotting analysis of Anti-DENV NS5 Antibody [Clone 37-2]
Virus infection Vero cell lysates were stained with mouse anti-DENV NS5
monoclonal antibody at 1:1000 dilution. Lane 1: Vero cell lysate (10 μg), Lane
2: ZIKV infection (10 μg), Lane 3: DENV1 infection (10 μg), Lane 4: DENV2
infection (10 μg), Lane 5: DENV3 infection (10 μg), Lane 6: DENV4 infection
(10 μg).

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Datasheet & Documents

Datasheet

MSDS

Manual

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