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Leadgene Leadgene
Anti-DENV NS5 Antibody [Clone 37-2]
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Catalog Number

LDG0007YA

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  • Overview

    Description

    Dengue virus belongs to the family Flaviviridae, genus Flavivirus and consists of four distinct serotypes (DENV1 to 4). Dengue virus is transmitted by mosquitos majorly of species Aedes aegypti and Aedes Albopictus that widespread around tropics and subtropics. NS5 is the largest and the most conserved protein of Dengue virus, acting as both a RNA-dependent RNA polymerase and a RNA methyltransferase enzyme. The NS5 proteins are important for viral replication and can down-regulate the host immune interferon response.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-5000
    IFA: 1:500-1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG2b

      Clone Name

      clone 37-2

    • Immunogen

      Dengue virus NS5

      Reactivity

      Dengue virus

    • Application

      ELISA, WB, IFA, FACS

      Conjugation

      Unconjugated

    • Concentration

      1 mg/mL

      Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Specificity

      NS5

      Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    Immunofluorescence analysis of Anti-DENV NS5 Antibody [Clone 37-2] Control Vero cells (uninfected) and Virus (DENV 1,2,4) infection Vero cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X- 100. Cells were stained with mouse anti-DENV NS5 monoclonal antibody followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    Immunofluorescence analysis of Anti-DENV NS5 Antibody [Clone 37-2]
    Control Vero cells (uninfected) and Virus (DENV 1,2,4) infection Vero cells
    were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-
    100. Cells were stained with mouse anti-DENV NS5 monoclonal antibody
    followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400,
    green) and cell nuclei were stained with Hoechst 33342 (Blue).

    ELISA titration of Anti-DENV NS5 Antibody [Clone 37-2] Titration curve of anti-DENV NS5 antibody in ELISA. Red: NS5; Black: BSA (negative control).

    ELISA titration of Anti-DENV NS5 Antibody [Clone 37-2]
    Titration curve of anti-DENV NS5 antibody in ELISA. Red: NS5; Black: BSA
    (negative control).

    Western blotting analysis of Anti-DENV NS5 Antibody [Clone 37-2] Virus infection Vero cell lysates were stained with mouse anti-DENV NS5 monoclonal antibody at 1:1000 dilution. Lane 1: Vero cell lysate (10 μg), Lane 2: ZIKV infection (10 μg), Lane 3: DENV1 infection (10 μg), Lane 4: DENV2 infection (10 μg), Lane 5: DENV3 infection (10 μg), Lane 6: DENV4 infection (10 μg).

    Western blotting analysis of Anti-DENV NS5 Antibody [Clone 37-2]
    Virus infection Vero cell lysates were stained with mouse anti-DENV NS5
    monoclonal antibody at 1:1000 dilution. Lane 1: Vero cell lysate (10 μg), Lane
    2: ZIKV infection (10 μg), Lane 3: DENV1 infection (10 μg), Lane 4: DENV2
    infection (10 μg), Lane 5: DENV3 infection (10 μg), Lane 6: DENV4 infection
    (10 μg).

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  • Datasheet & Documents
    MSDS
    Manual
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