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Catalog Number

LDG0009YA

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Overview

Description

Dengue virus belongs to the family Flaviviridae, genus Flavivirus and consists of four distinct serotypes (DENV1 to 4). Dengue virus is transmitted by mosquitos majorly of species Aedes aegypti and Aedes Albopictus that widespread around tropics and subtropics. The Membrane protein is the second smallest protein of dengue virus. Before dengue virus particles release, host protease furin cleaves prM into M. This amphipathic helical portion acts as an important component of the mature virion, interacting with envelope protein to promote invasion, packing mature, and release.

Product Note

Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-5000
IFA: 1:500-1000
FACS: Assay dependent

Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Specifications
- Host
  
  Mouse
  
  Clonality
  
  Monoclonal
- Isotype
  
  IgG2b
  
  Clone Name
  
  clone 31-1
- Immunogen
  
  Dengue virus M
  
  Reactivity
  
  Dengue virus
- Application
  
  ELISA, WB, IFA, FACS
  
  Conjugation
  
  Unconjugated
- Concentration
  
  1 mg/mL
  
  Buffer
  
  Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.
- Specificity
  
  M (membrane) protein
  
  Form
  
  Liquid
Instruction
- Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  Stability & Storage
  
  This product is stable after storage at:
  - 2-8°C for 2 weeks under sterile conditions from date of receipt.
  - -20°C or -80°C for 12 months under sterile conditions from date of receipt.
  Avoid repeated freeze/thaw cycles.
  Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.
Image

Immunofluorescence analysis of Anti-DENV M Antibody [Clone 31-1]
Control Vero cells (uninfected) and Virus (DENV 1,2) infection Vero cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with mouse anti-DENV M monoclonal antibody (1:400) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

Immunofluorescence analysis of Anti-DENV M Antibody [Clone 31-1]
Control BHK cells (uninfected) and Virus (DENV 1,2) infection BHK cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with mouse anti-DENV M monoclonal antibody (1:400) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

ELISA titration of Anti-DENV M Antibody [Clone 31-1]
Titration curve of anti-DENV M antibody in ELISA. Red: DENV M; Black: BSA (negative control).

Western blotting analysis of Anti-DENV M Antibody [Clone 31-1]
Virus infection C636 cell lysates were stained with mouse anti-DENV M monoclonal antibody at 1:1000 dilution.
Lane 1: ZIKV infection (10 μg), Lane 2: DENV1 infection (10 μg), Lane 3: DENV2 infection (10 μg), Lane 4: DENV3 infection (10 μg), Lane 5: DENV4 infection (10 μg), Lane 6: C636 cell lysate (10 μg).

Western blotting analysis of Anti-DENV M Antibody [Clone 31-1]
Virus infection Vero cell lysates were stained with mouse anti-DENV M monoclonal antibody at 1:1000 dilution.
Lane 1: Vero cell lysate (10 μg), Lane 2: ZIKV infection (10 μg), Lane 3: DENV1 infection (10 μg), Lane 4: DENV2 infection (10 μg), Lane 5: DENV3 infection (10 μg), Lane 6: DENV4 infection (10 μg).

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