Leadgene® 2X Fluorescent RT-LAMP Master Mix is an optimized master mix for reverse-transcription loop-mediated isothermal amplification (RT-LAMP) reactions. This product is a dual enzyme system, providing a rapid and sensitive detection in one pot. A fluorescent dye is also supplied with the kit. The LAMP reactions can be monitored through real-time fluorescence detection.
- Primer concentration
Primer concentration can be titrated between 0.25X - 1X if undesired background signal appeared.
- Reaction mixture preparation
Fluorescent dye should be freshly added to the reaction mixture.
2× Fluorescent RT-LAMP Master Mix
1 vial (1 mL)
50× LAMP Fluorescent Dye
1 vial (40 μL)