説明
This capping enzyme contains three enzymatic activities (RNA triphosphatase, guanylyltransferase and guanine methyltransferase) to forming a Cap 0 structure (m7Gppp5'N). In the presence of the vaccine capping enzyme, GTP and SAM (methyl donor) the In Vitro Transcripts mRNA can be efficient add capped in less one hour.
製品ノート
Reactionshould be performed under RNase free condition. Use nuclease-free tubes, reagents and water to avoid RNase contamination. Also, wear gloves when working with RNA.
コンポーネント
500 U
Items
Vaccinia Capping Enzyme
Quantity
1 vial (500 U)
Items
10X Capping Buffer
Quantity
1 vial (100 μL)