SUMO Protease (ULP1) (Active)

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カタログ番号

LDG0014RG

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• 概要

  説明

  SUMO Protease (ULP1, Ubiquitin-like-specific protease 1) is a highly active cysteine protease derived from Saccharomyces cerevisiae. It has often been used as a biotechnological tool for cleavage affinity purification tags such as ubiquitinlike (UBL) protein, and SUMO from fusion proteins. ULP1 protease specifically recognizes the tertiary structure of SUMO rather than an amino acid sequence. ULP1 protease has a His-tag for easy removal from a cleavage reaction by using nickel affinity resins. Notably, the cleavage reactions are available in a buffer
  containing 2 M urea.

  製品ノート

  • SUMO Protease (ULP1) protease: target protein ratio of 1U:2 µg is used for most fusion protein cleavage. Cleavage efficiency may differ based on structure and properties of each target protein, we recommend testing several enzyme-to-substrate ratios, temperatures, and incubation times.
  • SUMO Protease (ULP1) reactions can be performed in a buffer containing 2M urea.
  • SUMO Protease (ULP1) reactions can be performed in a buffer which is optimal for the target protein. Salts (e.g., NaCl) can be added to 300 mM for cleavage efficiency evaluation.

• 仕様

  • 発現系

    Escherichia coli

    濃度

    5 U/μL

  • 保存バッファー

    SUMO Protease is supplied in 25 mM Tris-HCl, 250 mM NaCl, 0.05% NP-40, 0.25 mM DTT, 50% glycerol, pH 8.0.

    純度

    >90% as determined by SDS-PAGE analysis.

  • 単位定義

    One unit of SUMO Protease (ULP1) cleaves > 85% of 2 μg control substrate in PBS at 30°C for 1 h.

    エンドトキシン量

    <1 EU per 1 μg of the protein by the LAL method.

  • 形状

    Liquid

• 使用説明

  • 配送

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    安定性および保存

    This product is stable after storage at:

    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.

• 画像

  SUMO Protease (ULP1) recognizes SUMO tertiary structure and cleaves at the C-terminal end of the Gly–Gly sequence in SUMO.

  SUMO Protease (ULP1) cleavage of control protein.

  SDS-PAGE analysis of recombinant SUMO Protease (ULP1)

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• 論文

  

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• データシート & ドキュメント

  データシート

  MSDS

  マニュアル

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免責事項：For Research Use or Further Manufacturing Only.