Streptavidin-HRP

カタログ番号

LDG0002RO

データシート

その他のドキュメント

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• 概要

  説明

  Streptavidin is a 53 kDa homotetramer isolated from Streptomyces avidinii. Because of its strong non-covalent interaction with biotin, streptavidin can be used to detect and isolate biotinylated proteins. Conjugation of horseradish peroxidase (HRP) to streptavidin is obtained by cross linking the amino groups on streptavidin with the carbohydrate groups on HRP. Streptavidin-HRP is typically used biotinylated antibodies or proteins.

  製品ノート

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-10000
  IHC: 1:200-500
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
  Horseradish peroxidase is conjugated to the streptavidin tetramer at about 1:1 molar ratio.
  Optimal titers for the applications should be determined by the researcher.

• 仕様

  • アプリケーション

    ELISA, WB, IHC

    コンジュゲーション

    HRP

  • 保存バッファー

    Liquid. Phosphate Buffered Saline containing 50% glycerol and 0.01% ProClin 300, pH 7.4.

    形状

    Liquid

• 使用説明

  • 配送

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    安定性および保存

    This product is stable after storage at:

    • 2-8°C for 6 months under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• レビュー

  

  この製品についてのご経験を共有してください。下のリンクから投稿いただけます。

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• 論文

  1. Tien SM et al. PLoS Pathog. 2022; 18(4):e1010469.

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• データシート & ドキュメント

  データシート

  MSDS

  マニュアル

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免責事項：For Research Use or Further Manufacturing Only.