RNase R

カタログ番号

LDG0027RG

その他のドキュメント

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COA 検索

• 概要

  説明

  Ribonuclease R (RNase R), derived from E. coli, is a magnesium-dependent exoribonuclease operating in a 3'→5' direction. Its primary function is to degrade linear RNAs while sparing lariat or circular RNA structures. Most cellular RNAs undergo complete digestion by RNase R, with exceptions such as tRNAs, 5S RNA, and intron lariats.
  RNase R effectively breaks down linear and Y-structured RNAs but does not affect lariats or circular RNAs. This property makes it useful for selectively enriching circular RNAs, which can be employed in protein production or intronic cDNA library construction.

  製品ノート

  • RNase R activity is optimal in the presence of low magnesium concentrations ranging from 0.1 to 1.0 mM. However, the effectiveness of RNase R can be hindered by low EDTA levels in RNA substrate solutions. To mitigate this negative impact, additional MgCl₂ can be added, up to a final concentration of 1 mM, to counteract the presence of EDTA in the substrate.
  • The enzyme exhibits its best performance at a temperature of 37°C.

  コンポーネント

  Package	Items	Quantity
  500 U	RNase R	1 vial (500 U)
  	10X Reaction buffer	1 vial (500 μL)

  • 500 U

    Items

    RNase R

    Quantity

    1 vial (500 U)

    Items

    10X Reaction buffer

    Quantity

    1 vial (500 μL)

• 仕様

  • 発現系

    Escherichia coli

    濃度

    20 U/ μL

  • 保存バッファー

    RNase R is supplied in a 50% glycerol solution containing 10 mM Tris, 250 mM KCl, 1 mM DTT, 0.1% Triton X-100, 0.1 mM EDTA, pH 7.6

    純度

    >95% as determined by SDS-PAGE analysis.

  • 単位定義

    One unit of RNase R converts 1 µg of poly(A) into acid-soluble nucleotides in 10 minutes at 37 °C under standard assay conditions.

    形状

    Liquid

• 使用説明

  • 配送

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    安定性および保存

    This product is stable after storage at:

    • -20°C for 12 months under sterile conditions from date of receipt.

• 画像

  Gel electrophoresis of poly(A) digested with RNase R.
  The standard assay was performed by incubating 1 μg of poly(A) with different unit of RNase R at 37°C for 10 minutes.

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  合計   (1)

• レビュー

  

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• 論文

  

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• データシート & ドキュメント

  データシート

  MSDS

  マニュアル

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免責事項：For Research Use or Further Manufacturing Only.