説明
Affinity purified Ni-NTA is conjugated to NHS-sepharose. It is an efficient technique for isolating recombinant proteins or other proteins. The Ni-NTA sepharose is specifically designed for the purification of recombinant proteins fused to the 6 x histidine (6 x His) tag expressed in bacteria, insects, and mammalian cells. The sepharose is high affinity and selectivity for recombinant fusion proteins that are tagged with six tandem histidine residues. The Ni-NTA resin can be used to purify 6 x His tagged proteins under native and denaturing conditions. Proteins bound to the resin can be eluted with low pH buffer or competition with imidazole or histidine.
製品ノート
The optimal protein concentration of the cell lysate should be determined by the end user.
