LEADSPHERE® Proteinase K

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カタログ番号

LDG0001RG

データシート

その他のドキュメント

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• 概要

  説明

  Proteinase K is a non-specific serine protease that belongs to the subtilisin family with an active site catalytic triad Asp39-His69-Ser224. It is useful for the general digestion of peptide bonds, consequently used in broad applications in the biology experiments such as isolation of genomic DNA and plasmid, isolation of RNA, inactivation of RNases, DNases, and enzymes in reactions.
  LEADSPHERE® Proteinase K sphere is competent in the digestion of samples in 300-400 μL, such as saliva. In addition, LEADSPHERE® Proteinase K is generated with an ISO13485 quality management system specifically for medical devices.

  製品ノート

  Please fine-tune the input sample volume to find the optimal condition for your assay.

  コンポーネント

  Package	Items	Quantity
  50 tablets	LEADSPHERE® Proteinase K	50 tablets
  	Proteinase K Diluent Buffer	1 vial (100 mL)

  • 50 tablets

    Items

    LEADSPHERE® Proteinase K

    Quantity

    50 tablets

    Items

    Proteinase K Diluent Buffer

    Quantity

    1 vial (100 mL)

• 仕様

  • アプリケーション

    Sample preparation

    純度

    >95% as determined by SDS-PAGE analysis.

  • 反応条件

    One tablet for one reaction, and one reaction can digest 16 μg BSA incubated with
    Proteinase K for 1 hour at 37o C.

    形状

    Lyophilized

• 使用説明

  • 配送

    The product is shipped at Ambient Temperature.

    安定性および保存

    This product is stable after storage at:

    • 4-30°C for 12 months under sterile conditions from date of receipt.

    Keep dry.

• 画像

  Lyophilized LEADSPHERE® Proteinase K

  Lyophilized LEADSPHERE® Proteinase K

  LEADSPHERE® Proteinase K pretreatment produces sensitive detection in RT-qPCR assay.

  LEADSPHERE™ Proteinase K completely digests bovine serum albumin (BSA).
  BSA was mixed with Proteinase K at 37 oC for an hour, and subsequently analyzed by SDS-PAGE. Lane 1. 2 μg of BSA Lane 2. 16 μg of BSA with 0.01 μg of Proteinase K Lane 3. 16 μg of BSA with 0.1 μg of Proteinase K Lane 4. 16 μg of BSA with 1 μg of Proteinase K

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  合計   (4)

• レビュー

  

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• 論文

  

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• データシート & ドキュメント

  データシート

  MSDS

  マニュアル

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免責事項：For Research Use or Further Manufacturing Only.