LeadGMP® Inorganic Pyrophosphatase

カタログ番号

LDG007R-GMP

データシート

その他のドキュメント

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COA 検索

• 仕様

  • 由来種

    Yeast

    発現系

    Escherichia coli

  • 濃度

    0.1 U/ μL

    保存バッファー

    20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 8.0

  • 純度

    >95% as determined by SDS-PAGE analysis.

    分子量

    The protein has a calculated MW of 33 kDa.
    The protein migrates as 35 kDa under reducing condition (SDS-PAGE analysis).

  • 活性

    One unit is the amount of enzyme that will generate 1 µmol of phosphate per minute from inorganic pyrophosphate (ppi) under standard reaction conditions
    (a 10 minute reaction at 25°C in 100 mM Tris-HCl, 2 mM MgCl2 and 2 mM PPi in a reaction volume of 0.5 mL, pH7.2).

    エンドトキシン量

    <0.1 EU per 1 μg of the protein by the LAL method.

  • マイコプラズマ

    Not detected

    形状

    Liquid

• 背景

  • 背景

    Inorganic pyrophosphatase (PPase) is an enzyme that catalyzes the hydrolysis of inorganic pyrophosphate (PPi) into two phosphate ions. This reaction is crucial for cellular phosphate metabolism and energy balance. In humans, the PPA1 gene encodes this enzyme, which is ubiquitously expressed across various tissues. PPases are essential in processes such as DNA synthesis, lipid metabolism, and calcium absorption. They are found in both prokaryotic and eukaryotic organisms, highlighting their fundamental role in biology.

• 使用説明

  • 配送

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    安定性および保存

    This product is stable after storage at:

    • -20°C for long-term storage under sterile conditions. Avoid repeated free-thaw cycles.

  • 製造仕様

    LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

    • Animal-free reagent and laboratory
    • Manufactured and tested under GMP guideline
    • Testing and traceability of raw material
    • Records of the maintenance and equipment calibration
    • Personnel training records
    • Batch-to-batch consistency
    • Documentation of QA control and process changes
    • Manufactured and tested under an ISO 13485:2016 certified quality management system
    • Stability monitor of product shelf-life

• 画像

  SDS-PAGE analysis of LeadGMP® Inorganic Pyrophosphatase.

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  合計   (1)

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• 論文

  

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• データシート & ドキュメント

  データシート

  MSDS

  マニュアル

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免責事項：For Research Use or Further Manufacturing Only.