LeadGMP® Human TNF Alpha Protein, His Tag, E. coli

カタログ番号

LDG014PHE-GMP

データシート

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仕様
- 由来種
  
  Human
  
  発現系
  
  Escherichia coli
- アフィニティタグ
  
  His Tag (C-term)
  
  保存バッファー
  
  Lyophilized from a 0.2 µm filtered solution of 500 mM NaCl PBS, pH 7.4.
- 純度
  
  >97% as determined by SDS-PAGE analysis.
  
  分子量
  
  The protein has a calculated MW of 18.3 kDa.
  The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).
- 活性
  
  Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED₅₀ for this effect is < 0.1 ng/mL. The specific activity of recombinant human TNF alpha is approximately ≧ 1 x 10⁷ IU/mg, which is calibrated against the human TNF Alpha WHO International Standard (NIBSC code: 12/154).
  
  エンドトキシン量
  
  <0.05 EU per 1 µg of the protein by the LAL method.
- マイコプラズマ
  
  Not detected
  
  形状
  
  Lyophilized
背景
- 背景
  
  Tumor necrosis factor alpha (TNF alpha) stimulates the acute phase of the immune response. In response to a pathogen, TNF alpha is one of the first to be released and can apply its effects in many organs. TNF alpha stimulates the release of corticotropic releasing hormone, suppresses appetite, and induces fever, in the hypothalamus. TNF increase vasodilation and loss of vascular permeability, it helps recruit lymphocyte, neutrophil, and monocyte to the inflammation site by regulating chemokine release.
  
  同義語
  
  Tumor necrosis factor, Cachectin, Tumor necrosis factor ligand superfamily member 2 , TNF-a
- Uniprot ID
  
  P01375
  
  配列ノート
  
  Val77-Leu233
使用説明
- 溶解
  
  It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
  
  配送
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
- 安定性および保存
  
  This product is stable after storage at:
  - -20°C for 12 months in lyophilized state from date of receipt.
  - -20°C or -80°C for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
  製造仕様
  
  LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:
  - Animal-free reagent and laboratory
  - Manufactured and tested under GMP guideline
  - Testing and traceability of raw material
  - Records of the maintenance and equipment calibration
  - Personnel training records
  - Batch-to-batch consistency
  - Documentation of QA control and process changes
  - Manufactured and tested under an ISO 13485:2016 certified quality management system
  - Stability monitor of product shelf-life
画像

The purity of LeadGMP® Human TNF Alpha Protein is greater than 95% as determined by RP-HPLC.

LeadGMP® Human TNF Alpha Protein, His Tag, E. coli (LDG014PHE-GMP) induce cytotoxicity in L929 cells with the ED₅₀ < 0.1 ng/mL.

SDS-PAGE analysis of LeadGMP® TNF alpha, Human

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合計 (3)
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データシート

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