LeadGMP® Human IL-7 Protein, His Tag, E. coli

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カタログ番号

LDG007PHE-GMP

データシート

その他のドキュメント

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• 仕様

  • 由来種

    Human

    発現系

    Escherichia coli

  • アフィニティタグ

    His Tag (C-term)

    保存バッファー

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • 純度

    >97% as determined by SDS-PAGE analysis.

    分子量

    The protein has a calculated MW of 18.3 kDa.
    The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis).

  • 活性

    Measured by its ability to induce PHA-activated human PBMCs proliferation. The ED₅₀ for this effect is <0.8 ng/mL. The specific activity of recombinant human IL-7 is > 1 x 10⁸ unit/mg, which is calibrated against the human IL-7 WHO Reference Reagent (NIBSC code: 90/530).

    エンドトキシン量

    <0.05 EU per 1 µg of the protein by the LAL method.

  • マイコプラズマ

    Not detected

    形状

    Lyophilized

• 背景

  • 背景

    Interleukin-7 (IL-7) is a multipotent cytokine belonged to one of the members of IL-2 superfamily. IL-7 has diverse effects on the hematopoietic and immune regulations. IL-7 is a trophic factor that is necessary for both B cell and T cell proliferation and development. In addition, it presents potential antitumor effects in tumors such as glioma, melanoma, lymphoma, leukemia, prostate cancer, and glioblastoma.

    同義語

    Interleukin-7

  • Uniprot ID

    P13232

    配列ノート

    Asp26-His177

• 使用説明

  • 溶解

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    配送

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • 安定性および保存

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

    製造仕様

    LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

    • Animal-free reagent and laboratory
    • Manufactured and tested under GMP guideline
    • Testing and traceability of raw material
    • Records of the maintenance and equipment calibration
    • Personnel training records
    • Batch-to-batch consistency
    • Documentation of QA control and process changes
    • Manufactured and tested under an ISO 13485:2016 certified quality management system
    • Stability monitor of product shelf-life

• 画像

  LeadGMP® Human IL-7 Protein, His Tag, E. coli (LDG007PHE-GMP) stimulates the proliferation of PHA-activated human peripheral blood lymphocytes (PBMC), with the ED50 < 0.8 ng/mL.

  SDS-PAGE analysis of LeadGMP® IL-7, Human

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  合計   (2)

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• 論文

  

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• データシート & ドキュメント

  データシート

  MSDS

  マニュアル

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免責事項：For Research Use or Further Manufacturing Only.