LeadGMP® Human IL-21 Protein, His Tag, E. coli

カタログ番号

LDG011PHE-GMP

データシート

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仕様
- 由来種
  
  Human
  
  発現系
  
  Escherichia coli
- アフィニティタグ
  
  His Tag (C-term)
  
  保存バッファー
  
  Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.
- 純度
  
  >97% as determined by SDS-PAGE analysis.
  
  分子量
  
  The protein has a calculated MW of 16.2 kDa.
  The protein migrates as 15-19 kDa under reducing condition (SDS-PAGE analysis).
- 活性
  
  Measure by its ability to enhance IFN gamma secretion in NK-92 cells. The ED₅₀ for this effect is <10 ng/mL.
  
  エンドトキシン量
  
  <0.05 EU per 1 µg of the protein by the LAL method.
- マイコプラズマ
  
  Not detected
  
  形状
  
  Lyophilized
背景
- 背景
  
  Interleukin-21 (IL-21) belongs to the IL-15/IL-21 family, which exerts pleiotropic immune regulations. IL-21 produced primarily by natural killer T (NKT) cells, T follicular helper (TFH) cells and TH17 cells. As a pleiotropic cytokine, IL-21 has been shown to regulate both innate and humoral immunity. It has potent inhibitory activity towards the activation and maturation of granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced dendritic cells (DCs). In B cells, IL-21 has a major role in the development of immunoglobulin responses. In T cells, it is required to facilitate the functional differentiation of several CD4+ T cell subsets. In addition, the ability of IL-21 to enhance the cytotoxic activity of both CD8+ T cells and NK cells makes it as a potential antitumor agent.
  
  同義語
  
  Interleukin-21, Za11
- Uniprot ID
  
  Q9HBE4
  
  配列ノート
  
  Gln32-Ser162
使用説明
- 溶解
  
  It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
  
  配送
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
- 安定性および保存
  
  This product is stable after storage at:
  - -20°C for 12 months in lyophilized state from date of receipt.
  - -20°C or -80°C for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
  製造仕様
  
  LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:
  - Animal-free reagent and laboratory
  - Manufactured and tested under GMP guideline
  - Testing and traceability of raw material
  - Records of the maintenance and equipment calibration
  - Personnel training records
  - Batch-to-batch consistency
  - Documentation of QA control and process changes
  - Manufactured and tested under an ISO 13485:2016 certified quality management system
  - Stability monitor of product shelf-life
画像

The purity of LeadGMP® Human IL-21 Protein is greater than 95% as determined by RP-HPLC.

LeadGMP® Human IL-21 Protein, His Tag, E. coli (LDG011PHE-GMP) enhances IFN gamma secretion in NK-92 cells, with the ED50 <10 ng/mL

SDS-PAGE analysis of LeadGMP® IL-21, Human

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合計 (3)
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データシート & ドキュメント

データシート

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