LeadGMP® Human IL-2 Protein, His Tag, E. coli

カタログ番号

LDG003PHE-GMP

データシート

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仕様
- 由来種
  
  Human
  
  発現系
  
  Escherichia coli
- アフィニティタグ
  
  His Tag (C-term)
  
  保存バッファー
  
  Lyophilized from a 0.2 µm filtered solution of NaPi buffer, 0.018% SDS, pH 7.5.
- 純度
  
  >97% as determined by SDS-PAGE analysis.
  
  分子量
  
  The protein has a calculated MW of 16.4 kDa.
  The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis).
- 活性
  
  Measure by its ability to induce proliferation in CTLL-2 cells. The ED₅₀ for this effect is <3 ng/mL. The specific activity of recombinant human IL-2 is >5 x 10⁶ IU/mg, which is calibrated against the human IL-2 WHO International Standard (NIBSC code: 86/500).
  
  エンドトキシン量
  
  <0.05 EU per 1 µg of the protein by the LAL method.
- マイコプラズマ
  
  Not detected
  
  形状
  
  Lyophilized
背景
- 背景
  
  Interleukin-2 (IL-2) is a 15.5 kDa four bundle, immuno-modulatory cytokine which is expressed predominately by antigen-simulated CD4+ T cells, CD8+ T cells, natural killer (NK) cells, and dendritic cells (DC). IL-2 plays a fundamental role in promoting NK cell proliferation, cytotoxicity, and B cells differentiation. IL-2 modulate T cell differentiation programs, promoting naïve CD4+ T cell differentiation into T helper-1 (Th1) and T helper-2 (Th2) cells.
  
  同義語
  
  Interleukin-2, T-cell growth factor , TCGF
- Uniprot ID
  
  P60568
  
  配列ノート
  
  Ala21-Thr153
使用説明
- 溶解
  
  It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
  
  配送
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
- 安定性および保存
  
  This product is stable after storage at:
  - -20°C for 12 months in lyophilized state from date of receipt.
  - -20°C or -80°C for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
  製造仕様
  
  LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:
  - Animal-free reagent and laboratory
  - Manufactured and tested under GMP guideline
  - Testing and traceability of raw material
  - Records of the maintenance and equipment calibration
  - Personnel training records
  - Batch-to-batch consistency
  - Documentation of QA control and process changes
  - Manufactured and tested under an ISO 13485:2016 certified quality management system
  - Stability monitor of product shelf-life
画像

The purity of LeadGMP® Human IL-2 Protein is greater than 95% as determined by SEC-HPLC.

LeadGMP® Human IL-2 Protein, His Tag, E. coli (LDG003PHE-GMP) induces CTLL-2 cell proliferation, with the ED50 < 3.0 ng/mL.

SDS-PAGE analysis of LeadGMP® IL-2, Human

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合計 (3)
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データシート & ドキュメント

データシート

MSDS

マニュアル

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