LeadGMP® Human IL-18 Protein, Tag Free, E. coli

カタログ番号

LDG025PHE-GMP

データシート

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仕様
- 由来種
  
  Human
  
  発現系
  
  Escherichia coli
- アフィニティタグ
  
  Tag Free
  
  保存バッファー
  
  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
- 純度
  
  >97% as determined by SDS-PAGE analysis.
  
  分子量
  
  The protein has a calculated MW of 18.2 kDa.
  The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).
- 活性
  
  Measure by its ability to induce IFN gamma secretion in KG-1 cells. The ED₅₀ for this effect is <6 ng/mL. The specific activity of recombinant LeadGMP® human IL-18 is > 1 x 10⁷ IU/mg, which is calibrated against the human IL-18 WHO Reference Reagent (NIBSC code: 03/200).
  
  エンドトキシン量
  
  <0.05 EU per 1 µg of the protein by the LAL method.
- マイコプラズマ
  
  Not detected
  
  形状
  
  Lyophilized
背景
- 背景
  
  Interleukin-18 (IL-18) belongs to the IL-1 superfamily and is constitutively produced by macrophages, dendritic cells (DCs) and other cells. IL-18 binds to the receptor of IL-18 (IL-18R) and initiate the recruitment and heterodimerization of the IL-18RAcP, leading to downstream activation of NF-κB. After stimulation with IL-18, multiple cytokines including IFN-γ, IL-13, IL-4, IL-8, and GM-CSF and both Th1 and Th2 lymphokines could be produced by different cells. As an immunoregulaory cytokine, IL-18 can promote development of T helper 1 (Th1) cells, which maximizes the production of IFN by synergistically stimulating to mature Th1 effectors in combination with IL-12. On the contrary, it inhibits the production of the anti-inflammatory cytokine IL-10. In addition, IL-18 exhibits multiple proinflammatory functions, such as increases in cell adhesion molecules, nitric oxide synthesis, and chemokine production.
  
  同義語
  
  Interleukin-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma , IL-1 gamma
- Uniprot ID
  
  Q14116
  
  配列ノート
  
  Tyr37-Asp193
使用説明
- 溶解
  
  It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
  
  配送
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
- 安定性および保存
  
  This product is stable after storage at:
  - -20°C for 12 months in lyophilized state from date of receipt.
  - -20°C or -80°C for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
  製造仕様
  
  LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:
  - Animal-free reagent and laboratory
  - Manufactured and tested under GMP guideline
  - Testing and traceability of raw material
  - Records of the maintenance and equipment calibration
  - Personnel training records
  - Batch-to-batch consistency
  - Documentation of QA control and process changes
  - Manufactured and tested under an ISO 13485:2016 certified quality management system
  - Stability monitor of product shelf-life
画像

The purity of LeadGMP® Human IL-18 Protein is greater than 95% as determined by RP-HPLC.

LeadGMP® Human IL-18 Protein, Tag Free, E. coli (LDG025PHE-GMP) induces IFN gamma secretion in KG-1 cells, with the ED50 < 6 ng/mL.

SDS-PAGE analysis of LeadGMP® IL-18, Human

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合計 (3)
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データシート & ドキュメント

データシート

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