LeadGMP® Human IFN Gamma Protein, His Tag, E. coli

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カタログ番号

LDG013PHE-GMP

データシート

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• 仕様

  • 由来種

    Human

    発現系

    Escherichia coli

  • アフィニティタグ

    His Tag (C-term)

    保存バッファー

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • 純度

    >97% as determined by SDS-PAGE analysis.

    分子量

    The protein has a calculated MW of 17.7 kDa.
    The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).

  • 活性

    Measure by its ability to induce cytotoxicity in HT29 cells. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant human IFN gamma is approximately >1 x 10⁷ IU/mg, which is calibrated against the human IFN Gamma WHO Reference Material (NIBSC code: 87/586).

    エンドトキシン量

    <0.05 EU per 1 µg of the protein by the LAL method.

  • マイコプラズマ

    Not detected

    形状

    Lyophilized

• 背景

  • 背景

    The cytokine IFN gamma could protect cells from viral infections and belongs to the family of interferons. A lot of studies have shown that IFN gamma secreted by antigen triggered cell types, including T cells, naive CD4+ T cells, macrophages, dendritic cells, and B cells. IFN gamma plays an important role to trigger the macrophage act against a diverse group of microbial targets, and the pleiotropic molecule associated with antiproliferative, pro-apoptotic and antitumor mechanisms. Based on the effector cytokine considered as a major effector of immunity, it has been used in the treatment of several diseases.

    同義語

    Interferon gamma, Immune interferon

  • Uniprot ID

    P01579

    配列ノート

    Gln24-Gln166

• 使用説明

  • 溶解

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    配送

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • 安定性および保存

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

    製造仕様

    LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

    • Animal-free reagent and laboratory
    • Manufactured and tested under GMP guideline
    • Testing and traceability of raw material
    • Records of the maintenance and equipment calibration
    • Personnel training records
    • Batch-to-batch consistency
    • Documentation of QA control and process changes
    • Manufactured and tested under an ISO 13485:2016 certified quality management system
    • Stability monitor of product shelf-life

• 画像

  LeadGMP® Human IFN Gamma Protein, His Tag, E. coli (LDG013PHE-GMP) induces cytotoxicity in HT29 cells, with the ED50 <1.0 ng/mL.

  SDS-PAGE analysis of LeadGMP® IFN gamma, Human

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• 論文

  

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• データシート & ドキュメント

  データシート

  MSDS

  マニュアル

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免責事項：For Research Use or Further Manufacturing Only.