LeadGMP® Human GM-CSF Protein, His Tag, E. coli

カタログ番号

LDG012PHE-GMP

データシート

その他のドキュメント

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COA 検索

• 仕様

  • 由来種

    Human

    発現系

    Escherichia coli

  • アフィニティタグ

    His Tag (N-term)

    保存バッファー

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • 純度

    >98% as determined by SDS-PAGE analysis.

    分子量

    The protein has a calculated MW of 15.4 kDa.
    The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).

  • 活性

    Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <80 pg/mL. The specific activity of recombinant human GM-CSF is approximately >1 x 10⁷ IU/mg.

    エンドトキシン量

    <0.05 EU per 1 µg of the protein by the LAL method.

  • マイコプラズマ

    Not detected

    形状

    Lyophilized

• 背景

  • 背景

    Granulocyte-macrophage colony-stimulating factor (GM-CSF) was first identified as a growth factor due to its ability to induce proliferation and differentiation of bone marrow progenitors into granulocytes and macrophages. GM-CSF is produced by multiple cell types including activated T cells, B cells, macrophages, endothelial cells and fibroblasts upon receiving immune stimuli. GM-CSF stimulates stem cells to produce granulocytes and monocytes functions as a cytokine.

    同義語

    Granulocyte-macrophage colony-stimulating factor, Colony-stimulating factor , CSF, Molgramostin, Sargramostim

  • Uniprot ID

    P04141

    配列ノート

    Ala18-Glu144

• 使用説明

  • 溶解

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    配送

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • 安定性および保存

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

    製造仕様

    LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

    • Animal-free reagent and laboratory
    • Manufactured and tested under GMP guideline
    • Testing and traceability of raw material
    • Records of the maintenance and equipment calibration
    • Personnel training records
    • Batch-to-batch consistency
    • Documentation of QA control and process changes
    • Manufactured and tested under an ISO 13485:2016 certified quality management system
    • Stability monitor of product shelf-life

• 画像

  SDS-PAGE analysis of LeadGMP® GM-CSF, Human

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  合計   (1)

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• 論文

  

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• データシート & ドキュメント

  データシート

  MSDS

  マニュアル

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免責事項：For Research Use or Further Manufacturing Only.