LeadGMP® Human EGF Protein, His Tag, E. coli

カタログ番号

LDG024PHE-GMP

データシート

その他のドキュメント

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COA 検索

• 仕様

  • 由来種

    Human

    発現系

    Escherichia coli

  • アフィニティタグ

    His Tag (C-term)

    保存バッファー

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • 純度

    >97% as determined by SDS-PAGE analysis.

    分子量

    The protein has a calculated MW of 7.16 kDa.
    The protein migrates as 9-11 kDa under reducing condition (SDS-PAGE analysis).

  • 活性

    Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <1.0 ng/mL. The specific activity of recombinant human EGF is approximately >1.0 x 10⁶ IU/mg.

    エンドトキシン量

    <0.05 EU per 1 µg of the protein by the LAL method.

  • マイコプラズマ

    Not detected

    形状

    Lyophilized

• 背景

  • 背景

    Human epidermal growth factor (EGF) is a 6 kDa cytokine with 53 amino acid residues. EGF is mainly secreted from ectodermal cells, monocytes, kidney and duodenal glands. Upon binding to its receptor, EGFR, EGF acts to stimulate cell growth and proliferation of epithelial cells, play important roles in many developmental processes including accelerate tooth eruption, inhibits gastric acid secretion, and involve in wound healing.

    同義語

    Epidermal Growth Factor, Urogastrone, URG

  • Uniprot ID

    P01133

    配列ノート

    Asn971-Arg1023

• 使用説明

  • 溶解

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    配送

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • 安定性および保存

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

    製造仕様

    LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

    • Animal-free reagent and laboratory
    • Manufactured and tested under GMP guideline
    • Testing and traceability of raw material
    • Records of the maintenance and equipment calibration
    • Personnel training records
    • Batch-to-batch consistency
    • Documentation of QA control and process changes
    • Manufactured and tested under an ISO 13485:2016 certified quality management system
    • Stability monitor of product shelf-life

• 画像

  SDS-PAGE analysis of LeadGMP® EGF, Human

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  合計   (1)

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• 論文

  

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• データシート & ドキュメント

  データシート

  MSDS

  マニュアル

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免責事項：For Research Use or Further Manufacturing Only.