LeadGMP® BspQI

カタログ番号

LDG003R-GMP

データシート

その他のドキュメント

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• 概要

  製品ノート

  • The volume of restriction endonuclease added should not exceed 1/10 of the reaction volume to avoid star activity.
  • DNA substrate should not contain phenol, chloroform, ethanol, EDTA, detergents, or high concentrations of salt, as these can affect the activity of BspQI enzyme.

  コンポーネント

  Specification	Item	Amount
  500 U	BspQI (10 U/μL)	(1 vial) 50 μL
  	10× R Buffer	(1 vial) 500 μL
  2500 U	BspQI (10 U/μL)	(1 vial) 250 μL
  	10× R Buffer	(2 vials) 1.25 mL
  10 KU	BspQI (10 U/μL)	(1 vial) 1 mL
  	10× R Buffer	(8 vials) 1.25 mL

  • 500 U

    Item

    BspQI (10 U/μL)

    Amount

    (1 vial) 50 μL

    Item

    10× R Buffer

    Amount

    (1 vial) 500 μL

  • 2500 U

    Item

    BspQI (10 U/μL)

    Amount

    (1 vial) 250 μL

    Item

    10× R Buffer

    Amount

    (2 vials) 1.25 mL

  • 10 KU

    Item

    BspQI (10 U/μL)

    Amount

    (1 vial) 1 mL

    Item

    10× R Buffer

    Amount

    (8 vials) 1.25 mL

• 仕様

  • 発現系

    Escherichia coli

    濃度

    10 U/μL

  • 保存バッファー

    20 mM Tris-HCl, 500 mM KCl, 0.1 mM EDTA, 1 mM DTT, 500 μg/ml rAlbumin, 50% Glycerol, 0.1% Triton X-100, pH 7

    活性

    One unit of BspQI is defined as the amount of enzyme that cleave 1 μg λDNA in a total reaction volume of 50 μL at 50°C for 1h.

  • エンドトキシン量

    <0.1 EU per 1 mL of the enzyme by the LAL method.

    マイコプラズマ

    Not detected

  • 形状

    Liquid

• 背景

  • 背景

    BspQI is a restriction enzyme derived from bacteria that recognizes and cuts specific DNA sequences. Its recognition sequence is 5'-GCTCTTC-3', with the cleavage site located downstream from the recognition sequence. BspQI is commonly used in molecular biology applications such as genome editing, cloning, and DNA mapping due to its ability to precisely cut DNA at defined sites, facilitating further analysis and manipulation of the DNA molecules.

• 使用説明

  • 配送

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    安定性および保存

    This product is stable after storage at:

    • -20°C for long-term storage under sterile conditions.

  • 製造仕様

    LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

    • Animal-free reagent and laboratory
    • Manufactured and tested under GMP guideline
    • Testing and traceability of raw material
    • Records of the maintenance and equipment calibration
    • Personnel training records
    • Batch-to-batch consistency
    • Documentation of QA control and process changes
    • Manufactured and tested under an ISO 13485:2016 certified quality management system
    • Stability monitor of product shelf-life

• 画像

  The enzyme activity of BspQI
  In a 50 μL reaction system, 1 μg of λDNA was treated with the 1U/μL of BspQI. The reaction was incubated at 50 °C for 60 minutes, followed by incubation at 80 °C for 20 minutes to inactivate BspQI.
  M: DNA marker
  Uncut: The control group was not treated with BspQI.
  BspQI disgest: The sample group was treated with BspQI.

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• 論文

  

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• データシート & ドキュメント

  データシート

  MSDS

  マニュアル

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免責事項：For Research Use or Further Manufacturing Only.