カタログ番号

LDG0037RG

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概要

説明

L–α–Glycerophosphate Oxidase (GPO) is a flavin-dependent enzyme that catalyzes the oxidation of sn-glycerol-3-phosphate to dihydroxyacetone phosphate (DHAP), with the production of hydrogen peroxide (H₂O₂) as a byproduct. This reaction plays an essential role in glycerol metabolism, serving as a critical link between lipid catabolism, glycolysis, and lipid biosynthesis through the generation of DHAP—a central intermediate in energy and metabolic pathways. In clinical and biochemical applications, GPO is frequently used in enzymatic assays for the quantification of triglycerides and glycerol. The hydrogen peroxide produced is coupled with a colorimetric reaction, allowing for easy detection and measurement. Due to its specificity and efficiency, GPO plays a vital role in analytical biochemistry and is a useful tool in both clinical laboratories and metabolic research.
仕様
- 発現系
  
  Escherichia coli
  
  検出方法
  
  Spectrophotometry
- 濃度
  
  10 U/mg or more
  
  活性
  
  Activity can be calculated by using the following formula:
  Volume activity (U/ mL)＝ ΔOD/ min (ΔOD test−ΔOD blank) × Vt × 2 × df /(13.2×1.0×Vs)
  ＝ ΔOD/ min × 4.696 × df
  Weight activity (U/mg)＝ (U/mL)×1/C
  Vt: Total volume (1.55 mL)
  Vs: Sample volume (0.05 mL)
  13.2: Millimolar absorption coefficient of quinoneimine dye
  2: Conversion factor (one mole of quinoneimine dye corresponds to two moles of glycerophophate)
  1.0: Light path length (cm)
  df: Dilution factor
  C: Enzyme concentration in dissolution (mg/mL)
- 単位定義
  
  One unit is defined as the enzyme quantity which oxidizes one micromole of L-α-Glycerol-phosphate per minute under the following conditions: 84 mM Potassium phosphate, 425 mM L-α-Glycerol-phosphate, 0.03% 4-Aminoantipyrine, 0.024% Phenol, and 1.9 U/mL Peroxidase.
  
  反応条件
  
  84 mM Potassium phosphate, 425 mM L-α-Glycerol-phosphate, 0.03% 4-Aminoantipyrine, 0.024% Phenol, and 1.9 U/mL Peroxidase
- 形状
  
  Lyophilized
背景
- 同義語
  
  GPO, Glycerol phosphate oxidase, α-Glycerophosphate oxidase, L–α–Glycerol-3-phosphate oxidase, G3P oxidase, Flavin-dependent oxidase, FAD oxidoreductase, Glycerophosphate:oxygen oxidoreductase, L–α–Glycerophosphate:oxygen oxidoreductase, Glycerol phosphate oxygenase, Aerococcus oxidase, Corynebacterium glycerophosphate oxidase, EC 1.1.3.21 enzyme
使用説明
- 溶解
  
  It is recommended to weigh and reconstitute 10 mg of lyophilized powder in 125 μL double-distilled water directly and incubate the solution for at least 10 mins to ensure sufficient re-dissolved.
  
  配送
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
- 安定性および保存
  
  This product is stable at -20°C for long-term storage under sterile conditions.
  Avoid repeated free-thaw cycles.
画像

The effects of various chemicals on GPO.
The enzyme was incubated with the chemicals at 25°C for 1 hour

pH stability of GPO.
The enzyme powder was reconstituted by double-distilled water and treated with different pH buffer condition for 20 hours. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

Thermal stability of GPO.
The enzyme powder was reconstituted by double-distilled water and treated with different temperatures for 15 minutes. Final concentration: 21.6 U/ mL.

pH activity of GPO.
The buffer conditions with various pH values were used in the reaction at 37°C. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

Temperature activity of GPO.
The enzyme reactions in 0.2 M KH2PO4-NaOH, pH 7.0, were carried out under different temperatures.

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