説明
IdeZ Protease is a recombinant enzyme originally isolated from Streptococcus equi subspecies zooepidemicus that cleaves IgG at a specific site in the lower hinge region. It serves as a complementary tool to IdeZ by offering broader specificity, effectively digesting mouse IgG2a and IgG3 subclasses which are typically resistant to other enzymes.
発現系
Escherichia coli
保存バッファー
Lyophilized from a 0.2 µm filtered solution of 50 mM Sodium phosphate, 150 mM NaCl, pH 6.6.
純度
>95% as determined by SDS-PAGE analysis.
分子量
The protein has a calculated MW of 36.44 kDa.
The protein migrates as 35-36 kDa under reducing condition (SDS-PAGE analysis).
活性
50 U/µL
単位定義
One unit is defined as the amount of enzyme required to cleave ≥ 95% of 1 µg recombinant human IgG in 30 minutes at 37°C.
エンドトキシン量
<0.1 EU per 1 μg of the protein by the LAL method.
形状
Lyophilized
同義語
IgG-degrading enzyme Z, IgG cleaving enzyme, Immunoglobulin G–specific protease Z, Streptococcal protease IdeZ, Endopeptidase IdeZ, IgG hinge-specific protease
溶解
It is recommended to reconstitute the lyophilized protein in sterile H₂O and incubate the stock solution for at least 10 min to ensure sufficient re-dissolved.
配送
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
安定性および保存
This product is stable after storage at -20°C for 12 months in lyophilized state from date of receipt. Upon reconstitution, protein aliquots should be stored at 4°C.
SDS-PAGE analysis of recombinant human IgG digested with IdeZ protease.
The standard assay was performed by incubating 2 units of IdeZ protease and 2 μg of recombinant human IgG at 37°C for 30 minutes.
SDS-PAGE analysis of IdeZ Protease.
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免責事項：For Research Use or Further Manufacturing Only.
