説明
IdeS Protease is a highly specific cysteine protease derived from Streptococcus pyogenes that cleaves human IgG antibodies at a single site in the lower hinge region. This enzyme is essential for therapeutic antibody and Fc fusion protein characterization, enabling the rapid generation of homogenous F(ab')2 and Fc fragments for detailed analysis via mass spectrometry or chromatography.
発現系
Escherichia coli
保存バッファー
Lyophilized from a 0.2 µm filtered solution of 50 mM Sodium phosphate, 150 mM NaCl, pH 6.6.
純度
>95% as determined by SDS-PAGE analysis.
分子量
The protein has a calculated MW of 36.22 kDa.
The protein migrates as 32-35 kDa under reducing condition (SDS-PAGE analysis).
活性
70 U/µL
単位定義
One unit is defined as the amount of enzyme required to cleave ≥ 95% of 1 µg recombinant human IgG in 30 minutes at 37°C.
エンドトキシン量
<0.1 EU per 1 μg of the protein by the LAL method.
形状
Lyophilized
同義語
IgG-degrading enzyme of Streptococcus pyogenes, Endopeptidase IdeS, IgG-specific cysteine protease, Fab/Fc cleaving protease
溶解
It is recommended to reconstitute the lyophilized protein in sterile H₂O and incubate the stock solution for at least 10 min to ensure sufficient re-dissolved.
配送
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
安定性および保存
This product is stable after storage at -20°C for 12 months in lyophilized state from date of receipt. Upon reconstitution, protein aliquots should be stored at 4°C.
SDS-PAGE analysis of recombinant human IgG digested with IdeS protease.
The standard assay was performed by incubating 2 units of IdeS protease and 2 μg of recombinant human IgG at 37°C for 30 minutes.
SDS-PAGE analysis of IdeS Protease.
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免責事項：For Research Use or Further Manufacturing Only.
