HyLink™ BirA Biotin Labeling Kit

もっと見る

カタログ番号

LDG0021RC

データシート

その他のドキュメント

パッケージと価格を読み込み中...

COA 検索

• 概要

  説明

  The HyLink™ BirA Biotin Labeling Kit for Avi-tag Proteins provides a highly efficient, specific, and reproducible solution for biotinylating recombinant proteins or peptides containing Avi-tags or other enzymatic biotinylation tags such as Biotag™. This kit utilizes the biotin ligase enzyme BirA, which specifically and uniformly attaches biotin to a lysine residue within the Avi-tag sequence. Compared to traditional chemical biotinylation methods, this enzymatic approach offers site-specific labeling, minimizes variability between batches, and preserves the biological activity and structural integrity of the target protein.

  コンポーネント

  Item	Quantity	Storage
  GST-BirA (lyophilized)	40 μg, 1 vial	Store at -20°C
  Reconstitution buffer	0.2 mL, 1 vial	Store at -20°C
  10X Reaction buffer	1.5 mL, 1 vial	Store at -20°C
  SpinDesalt Column	16 pcs	Store at 4°C

  • GST-BirA (lyophilized)

    Quantity

    40 μg, 1 vial

    Storage

    Store at -20°C

  • Reconstitution buffer

    Quantity

    0.2 mL, 1 vial

    Storage

    Store at -20°C

  • 10X Reaction buffer

    Quantity

    1.5 mL, 1 vial

    Storage

    Store at -20°C

  • SpinDesalt Column

    Quantity

    16 pcs

    Storage

    Store at 4°C

• 使用説明

  • 配送

    The product is shipped with polar packs. Upon receipt, store the components under the recommended storage conditions immediately.

    安定性および保存

    This product is stable under the following storage conditions:

    • The enzyme and buffer are stable for up to 12 months when stored at −20 °C under sterile conditions from the date of receipt.
    • The column is stable for up to 12 months when stored at 4 °C from the date of receipt.

    Avoid repeated freeze–thaw cycles.

• 画像

  The biotinylation efficiency of proteins was analyzed by (A) SDS-PAGE and (B) Western blot using Streptavidin-HRP as a probe.
  Lane 1: Biotin unconjugated proteins
  Lane 2: Biotin conjugated proteins using LDG0021RC

  Protein biotinylation efficiency was analyzed using a Streptavidin gel shift assay.
  Lane 1: Biotin-conjugated proteins, without Streptavidin
  Lane 2: Biotin-conjugated proteins, with Streptavidin

  1

  /

  2

  合計   (2)

• レビュー

  

  この製品についてのご経験を共有してください。下のリンクから投稿いただけます。

  レビューを投稿

• 論文

  

  現在論文はありません。下のリンクからお知らせください。

  論文を投稿

• データシート & ドキュメント

  データシート

  MSDS

  マニュアル

  1

  /

  2

免責事項：For Research Use or Further Manufacturing Only.