Hot Start Taq DNA Polymerase (Glycerol-free)

カタログ番号

LDG0010RF

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• 概要

  説明

  Leadgene® Hot Start Taq DNA Polymerase contains Taq DNA Polymerase and an anti-taq monoclonal antibody which blocks polymerase activity. Enzyme activity is recovered during the initial incubation step while the taq antibody is denatured and dissociates from the DNA polymerase. Hot Start Taq DNA Polymerase exhibits higher specificity, sensitivity, and yield by reducing non-specific amplification and primer-dimers. This enzyme possesses 5’→3’ polymerase activity and 5’ → 3’ exonuclease replacement activity, but lacks a 3’ → 5’ exonuclease (proof-reading) activity. Hot Start Taq DNA Polymerase is suitable for most PCR applications. The enzyme formulation does not contain glycerol and is compatible for further lyophilization process.

  コンポーネント

  Package	Items	Quantity
  500 U	Hot Start Taq DNA Polymerase (Glycerol-Free) (5 U/μL)	1 vial (500 U)
  	10× Hot Start Taq Buffer (Mg²⁺ free)	1 vial (1 mL)
  	25 mM MgCl₂	1 vial (1 mL)
  1,000 U	Hot Start Taq DNA Polymerase (Glycerol-Free) (5 U/μL)	1 vial (1,000 U)
  	10× Hot Start Taq Buffer (Mg²⁺ free)	2 vials (1 mL)
  	25 mM MgCl₂	2 vials (1 mL)

  • 500 U

    Items

    Hot Start Taq DNA Polymerase (Glycerol-Free) (5 U/μL)

    Quantity

    1 vial (500 U)

    Items

    10× Hot Start Taq Buffer (Mg²⁺ free)

    Quantity

    1 vial (1 mL)

    Items

    25 mM MgCl₂

    Quantity

    1 vial (1 mL)

  • 1,000 U

    Items

    Hot Start Taq DNA Polymerase (Glycerol-Free) (5 U/μL)

    Quantity

    1 vial (1,000 U)

    Items

    10× Hot Start Taq Buffer (Mg²⁺ free)

    Quantity

    2 vials (1 mL)

    Items

    25 mM MgCl₂

    Quantity

    2 vials (1 mL)

• 仕様

  • アプリケーション

    RT-PCR, qPCR

    濃度

    5 U/µL

  • 純度

    >98% as determined by SDS-PAGE analysis.

    単位定義

    One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C.

  • 形状

    Liquid

• 使用説明

  • 配送

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    安定性および保存

    This product is stable after storage at:

    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.

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• データシート & ドキュメント

  データシート

  MSDS

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免責事項：For Research Use or Further Manufacturing Only.